Background:Previously,we showed that total flavonoids from astragalus (TFA) had beneficial effects against transforming growth factor (TGF)-β1-mediated fibrosis,but whether these effects involved autophagy is not known.We attempt to explore the effects of TFA on autophagy in an animal model of idiopathic pulmonary fibrosis (IPF) induced by bleomycin,and to look for TFA components that may have an effect on autophagy.Methods:C57BL/6 mice were randomized to the sham group (SG),model group,low-dose TFA group (LDTG),and high-dose of TFA group (HDTG).The A549 cell line was treated with the TFA components including formononetin,calycosin,isorhamnetin,kaempferol,and quercetin.Lung tissues and cells were examined by histology,immunohistochemistry (anti-TGF-β1,α-smooth muscle actin (SMA),and cadherin),immunofluorescence (microtubule-associated protein light chain 3 (LC3)),hydroxyproline content,and immunoblotting (smad3,smad7,phosphoinositide 3-kinase (PI3K),α-SMA,E-cadherin,beclin-1,and LC3-Ⅱ).Results:In vivo,TFA inhibited TGF-β1 expression and decreased collagen content in lung tissues induced by bleomycin.TFA increased autophagy following suppression of the smad pathway.In vitro,quercetin inhibited the epithelial-mesenchymal transition (EMT) of A549 cells induced by TGF-β1 through suppression of the smad pathway.Autophagy was also increased by quercetin through inhibition of the AKT/mTOR pathway,but without change in PI3K expression.Formononetin,calycosin,isorhamnetin,and kaempferol had no such effects.Conclusion:TFA can alleviate bleomycin-induced PF in C57BL/6 mice via enhanced autophagy.The smad and AKT/mTOR pathways are possibly involved in these effects.Quercetin was the main active compound in TFA.