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复合性状转基因玉米Bt11×GA21是孟山都公司研发的新品种。目前,由于复合性状转基因作物没有特异的侧翼序列,所以没有合适的方法来准确检测复合性状转基因作物。本研究开发了一种新型通用引物多重PCR技术(UP-M-PCR),可以同时检测5种目标序列(NOS、35S、BT11、GA21和IVR),实现快速筛选检测Bt11×GA21玉米(Zea mays L.)。与传统多重PCR检测技术相比,此新型技术克服了传统方法固有的一些缺点,如体系复杂,低灵敏度,自抑制和非特异扩增等。本研究首先设计了引物(包括通用引物UP和带接头的特异性引物GA21-466-F/R,Bt11-277-F/R,NOS-216-F/R,IVR-175-F/R,35S-137-F/R);含接
Compound trait genetically modified corn Bt11 × GA21 is Monsanto company developed a new breed. At present, there is no suitable method for accurately detecting trait genetically modified crops because there is no specific flanking sequence for the trait gene. In this study, a new universal primer multiplex PCR (UP-M-PCR) was developed to detect five target sequences (NOS, 35S, BT11, GA21 and IVR) simultaneously and achieve rapid screening of Bt11 × GA21 maize L.). Compared with the traditional multiplex PCR detection technology, this new technology overcomes some inherent disadvantages of the traditional methods, such as complex system, low sensitivity, self-inhibition and non-specific amplification. In this study, we designed primers (including general primers UP and specific primers GA21-466-F / R, Bt11-277-F / R, NOS- 216-F / R, IVR- 175-F / R, 35S-137-F / R); with access