目的 :将人内皮抑素 (endostatin)基因插入表达载体并进行原核表达。方法 :将 endostatin基因定向插入表达载体 p QE-31Bam H I/ Kpn I位点 ,构建重组质粒 p QEN,转化 E.coli M15 ,进行原核表达。结果 :在 IPTG的诱导下 ,endostatin基因在重组转化株 E.coli M15 (p QEN)中获得高效表达 ,SDS- PAGE显示表达产物分子量为 2 2× 10 3u,表达产物主要以包涵体形式存在 ,表达量占菌体总蛋白的 34 .6 %。结论 :从组织中克隆 endostatin基因并进行原核表达是可行的方法 Objective: To insert human endostatin gene into expression vector and prokaryotic expression. Methods: The endostatin gene was inserted into the expression vector pQE-31Bam H I / Kpn I site to construct the recombinant plasmid pQEN. The recombinant plasmid was transformed into E. coli M15 for prokaryotic expression. RESULTS: The endostatin gene was highly expressed in the recombinant E. coli M15 (pQEN) induced by IPTG. SDS-PAGE showed that the molecular weight of the endostatin gene was 22 × 10 3 u, and the expression product was mainly in the form of inclusion body. The expression amount accounted for 34.6% of the total bacterial protein. Conclusion: It is feasible to clone endostatin gene from the tissue and prokaryotic expression