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研究10(男7,女3)例经活检证实的特发性闭塞性细支气管炎伴机化性肺炎(BOOP)患者,平均年龄55岁,胸片均显示双侧斑片状浸润。用支气管肺泡灌洗液(BALF)的细胞学和免疫细胞学检测结果,与以下易误诊的间质性肺疾病患者作比较,并以11名健康不吸烟受试者作对照。特发性肺纤维化(IPF)22(男12、女10)例,平均年龄52岁,开胸肺活检示间质性肺炎。慢性嗜酸细胞性肺炎(CEP)9(男 3、女6)例,平均年龄44岁,根据典型标准作出诊断。外源性变应性肺泡炎(EAA)24(男 11、女 13)例,平均年龄48岁,根据病史、临床和放射学特征等诊断。BAL是用0.9%盐水100ml分5等价滴入受累肺段,如阴影弥散时则滴入中叶。经消毒纱布过滤BALF,测回收量,计算总细胞数和细胞分类,分类时至少要计数600个细胞,用免疫过氧化物酶玻片法经表面标记物分析检测淋巴细胞亚群。
Study 10 (7 males and 3 females) A biopsy confirmed idiopathic bronchiolitis obliterans with opportunistic pneumonitis (BOOP), mean age 55 years, showed bilateral patchy infiltrates on the chest radiograph. The results of cytology and immunocytochemistry of bronchoalveolar lavage fluid (BALF) were compared with the following easily misdiagnosed patients with interstitial lung disease and compared with 11 healthy non-smoking subjects. Idiopathic pulmonary fibrosis (IPF) 22 (male 12, female 10) cases, mean age 52 years, open thoracic biopsy showed interstitial pneumonia. Chronic eosinophilic pneumonia (CEP) 9 (3 males and 6 females) with a mean age of 44 years and diagnosed according to typical criteria. Allergic alveolitis (EAA) 24 (11 males and 13 females) with a mean age of 48 years was diagnosed on the basis of medical history, clinical and radiological features. BAL is 0.9% saline 100ml points 5 equivalent drops into the affected lung segment, such as when the shadow of dispersion is dripped in the middle. BALF was sterilized by sterile gauze, and the total amount of cells and cell category were calculated. At least 600 cells should be counted in the classification. Lymphocyte subsets were detected by surface marker analysis using the immunoperoxidase slide method.