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目的探讨白介素-27(interleukin-27,IL-27)对中枢神经系统(central nervous system,CNS)炎性趋化因子(chemokine RANTES,CCL5)的调节作用,以及在小鼠实验性自身免疫性脑脊髓膜炎(experimental autoimmune encephalomyelitis,EAE)中的治疗效果。方法 40只雌性C57BL/6小鼠建模后,分别于第0、7、14、21天用Western blot检测CNS中IL-27和CCL5的变化。另将50只小鼠随机分为正常组、EAE组、EAE+IL-27组、EAE+SC144(IL-27受体抑制剂)组和EAE+PBS组,定期观察动物行为变化,并在建模后21 d检测CCL5的表达。结果建模后,IL-27在CNS中的表达随时间逐渐升高,而CCL5的表达随时间先升高后下降。经IL-27干预后,小鼠神经功能缺损较对照减轻(P<0.05),而SC144组小鼠神经功能缺损较其他组加重(P<0.05)。在21 d,CCL5在IL-27干预组的表达较EAE模型组明显降低(P<0.05),而在SC144组较EAE模型组显著增高(P<0.05)。结论在EAE模型中,IL-27抑制CCL5的表达,从而发挥抗炎作用减轻小鼠神经功能缺损。
Objective To investigate the regulatory effect of interleukin-27 (IL-27) on chemokine RANTES (CCL5) in the central nervous system (CNS) and its role in the experimental autoimmune brain Therapeutic effect in experimental autoimmune encephalomyelitis (EAE). Methods After the establishment of 40 female C57BL / 6 mice, the changes of IL-27 and CCL5 in the CNS were detected by Western blot on days 0, 7, 14 and 21, respectively. Another 50 mice were randomly divided into normal group, EAE group, EAE + IL-27 group, EAE + SC144 (IL-27 receptor inhibitor) group and EAE + PBS group. After 21 days, the expression of CCL5 was detected. Results After modeling, the expression of IL-27 in CNS gradually increased with time, while the expression of CCL5 increased first and then decreased with time. After IL-27 intervention, the neurological deficits in mice were relieved (P <0.05), while those in SC144 group were more severe than those in other groups (P <0.05). At 21 days, the expression of CCL5 in IL-27 intervention group was significantly lower than that in EAE model group (P <0.05), but significantly higher in SC144 group than EAE model group (P <0.05). Conclusion In EAE model, IL-27 inhibits the expression of CCL5, thereby exerting anti-inflammatory effects and reducing neurological deficits in mice.