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目的研究强啡肽Ａ（ＤｙｎＡ１～１３）与大鼠胶原酶诱导的脑出血后脑水肿形成的关系，寻找治疗脑出血后脑水肿的特异性药物。方法大鼠大脑尾壳核内微量注射０．４ＩＵ细菌胶原酶；４ｈ后侧脑室注射ＤｙｎＡ１～１３及κ受体拮抗剂ｎｏｒ－ＢＮＩ；放射免疫法测定脑出血大鼠皮层、海马、尾壳核、下丘脑和垂体中ＤｙｎＡ１～１３含量；干湿重法测定脑组织含水量。结果胶原酶诱导大鼠脑出血后，顶叶皮层、额叶皮层、海马和尾壳核内ＤｙｎＡ１～１３含量明显减少，与对照组相比，Ｐ＜０．０５，Ｐ＜０．０１，与此同时，上述脑区组织含水量亦明显增加；但下丘脑和垂体ＤｙｎＡ１～１３含量明显增加（Ｐ＜０．０１）。侧脑室给予ＤｙｎＡ１－１３可以显著降低脑出血后脑组织含水量；κ受体拮抗剂ｎｏｒ－ＢＮＩ可以阻断该作用。结论ＤｙｎＡ１～１３可以通过κ受体治疗脑出血后脑水肿。
Objective To study the relationship between dynorphin A (DynA1 ~ 13) and cerebral collagenase-induced cerebral edema induced by intracerebral hemorrhage in rats and to find out the specific drugs for cerebral edema after intracerebral hemorrhage. Methods After injection of 0.4IU bacterial collagenase into the caudate putamen, rats were injected with DynA1 ~ 13 and nor-BNI, and the radioimmunoassay was used to measure the cortex, hippocampus and caudate putamen , DynA1 ~ 13 content in the hypothalamus and pituitary gland; Results After intracerebral hemorrhage induced by collagenase, the content of DynA1 ~ 13 in parietal cortex, frontal cortex, hippocampus and caudate putamen decreased significantly compared with the control group (P <0.05, P <0.01) At the same time, the water content of brain tissue also increased significantly, but the content of DynA1 ~ 13 in hypothalamus and pituitary significantly increased (P <0.01). Lateral ventricular administration of DynA1-13 can significantly reduce brain water content after intracerebral hemorrhage; κ-receptor antagonist nor-BNI can block this effect. Conclusions DynA1 ~ 13 can treat cerebral edema after intracerebral hemorrhage via κ receptor.