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目的建立康力宝口服液高效液相色谱指纹图谱检测方法。方法以麦角甾苷为参照物,应用高效液相色谱法(HPLC),色谱柱为Shi m-pack VP-ODS柱(4.6 mmID×150 mm,粒径5μm),流动相为甲醇-乙腈-四氢呋喃-0.1 mol/L醋酸钠缓冲液(8∶8∶7∶77,冰醋酸调pH至4.0);流速1.0 ml/min,检测波长334 nm。结果标示出康力宝口服液13个共有峰。结论该方法简便、准确、重现性好,为康力宝口服液指纹图谱质量控制提供了依据。
Objective To establish a method for the determination of Kangli Bao oral liquid by high performance liquid chromatography (HPLC). Methods Using acteoside as a reference substance, the column was identified as Shi m-pack VP-ODS column (4.6 mmID × 150 mm, particle size 5 μm) by high performance liquid chromatography (HPLC). The mobile phase consisted of methanol-acetonitrile- -0.1 mol / L sodium acetate buffer (8: 8: 7: 77, adjusted to pH 4.0 with glacial acetic acid); flow rate 1.0 ml / min, detection wavelength 334 nm. The results indicate that there are 13 common peaks for the Kangli Bao oral solution. Conclusion The method is simple, accurate and reproducible. It provides the basis for the quality control of Kangli Bao oral solution.