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用荧光显微技术对王官溪蜜柚 (Citrusgrandis cv.Guanximiyou)和度尾蜜柚 (Citrusgrandiscv.Duweimiyou)自交和互交亲和性进行了观察 .发现两品种自交和互交花粉在柱头上都萌发正常 .自交花粉管进入柱头组织后 ,大部分在伸长过程出现破裂 ,伴随有内容物渗出 ,在花柱道内缘有胼胝质出现 ,但仍有部分花粉管能正常生长进入子房 ,并从胚柄处进入胚珠 ,在珠心组织里 ,观察到胼胝质的积累 ,其中大多数王官溪蜜柚和少数度尾蜜柚胚珠的胚囊位置出现强烈的荧光亮点 .而互交的胚珠中没有观察到这些现象 .由此可见 ,两品种的自交组合在花粉管进入柱头组织 ,直至进入子房、胚珠的整个伸长过程中 ,存在一系列不亲和反应
The selfing and reciprocal reciprocity of Citrusgrandis cv.Guanximiyou and Citrusgrandiscv. Duweimiyou were observed by fluorescence microscopy, and it was found that both varieties of selfing and reciprocal pollen Germination normal.After selfing pollen tube into the stigma tissue, most of the rupture occurred in the elongation process, accompanied by the contents of exudation, there is callose in the inner edge of the flowers appear, but there are still some pollen tube can grow normally into the ovary, And from the embryo into the ovule in the bead heart tissue, the accumulation of callose is observed, most of which Guanghui pomelo and a few degrees tail pomelo ovule embryo sac position strong fluorescence highlights, while the reciprocal ovule These phenomena were not observed.It can be seen that the selfing combination of two varieties entered the stigma tissue in the pollen tube and there was a series of unpaired reactions during the whole elongation of the ovary and ovule