目的观察芎芍胶囊对兔动脉粥样硬化模型脂质代谢及血小板聚集的影响并探讨其可能机制。方法用4F·Forgarty导管剥脱损伤腹主动脉内皮后继喂高胆固醇饲料的方法,复制节段性兔动脉粥样硬化模型。70只动物术后随机分为7组,即模型3天、2周、6周组(A、B、C组),单纯内皮损伤组(D组),普罗布考组(E组),芎芍胶囊小剂量组(F组),芎芍胶囊大剂量组(G组),另10只为假手术组(N组)。除D组和N组外,其余各组均在高胆固醇饲料基础上分别喂以不同种类及剂量的药物。在各观察时点,动物处死前心脏抽血测定TC、TG、HDL-C、LDL-C等指标;术后3天及实验结束时心脏取血,测定5 min内血小板最大聚集率(mPAGR)。结果(1)对AS兔血脂水平的影响:D组血脂水平与N组比较无明显变化(P>0.05);动物喂食高脂饲料3天后TC、LDL-C即明显升高,A、B、C组与N组比较差异均有统计学意义(P<0.05,P<0.01);实验结束时,与C组比较,G、E组TC、LDL-C均明显降低,G、E、F各组均能降低LDL-C/HDL-C比值,尤以G组明显(P<0.01);G组尚有升高HDL-C的趋势,E组变化不明显。G组可明显降低致动脉粥样硬化指数(AI,TC/HDL-C),与C、E组比较,差异有统计学意义(P<0.05)。(2)对血小板聚集的影响:内皮损伤后3天A、B、C组及D组mPAGR均明显升高,与N组比较差异均有统计学意义(P<0.01);G、E、F各组均明显降低术后3天mPAGR,与C组比较,G组差异有统计学意义(P<0.05)。G、E、F各组均可降低术后6周mPAGR,与C组比较有统计学意义(P<0.05,P<0.01),以G组作用更为显著。结论芎芍胶囊可降低兔动脉粥样硬化模型TC、LDL-C水平及动脉粥样硬化指数AI,还可抑制该模型的早期及术后6周血小板集聚,这些可能是其抗AS的机制之一。 Objective To observe the effect of Fuling capsule on lipid metabolism and platelet aggregation in rabbit atherosclerosis model and to explore its possible mechanism. Methods The segmental rabbit atherosclerosis model was created by peeling off the injured endothelium of abdominal aorta with 4F•Forgarty catheter and feeding high cholesterol diet. The 70 animals were randomly divided into 7 groups: the 3, 2 and 6 weeks (A, B, and C groups), the simple endothelium injury group (D), and the probucol group (E group). The capsules were given in small dose group (F group) and high dose group (G group), while the other 10 were sham group (N group). Except group D and group N, other groups were fed with different types and doses of drugs on the basis of high cholesterol diets. At each time point of observation, blood samples were taken from the heart to measure TC, TG, HDL-C, and LDL-C. At the end of the experiment and at the end of the experiment, blood was drawn from the heart and the maximum platelet aggregation rate (mPAGR) was measured within 5 minutes. . Results (1) Effect of blood lipid level on AS rabbits: There was no significant difference in serum lipid levels between group D and group N (P>0.05); TC and LDL-C levels were significantly increased after animals fed high-fat diet for 3 days, A, B, The difference between group C and group N was statistically significant (P<0.05, P<0.01). At the end of the experiment, compared with group C, the TC and LDL-C in group G and E were significantly lower, and each of G, E, and F was decreased. All groups were able to lower the ratio of LDL-C/HDL-C, especially in group G (P<0.01). G group also had the tendency of increasing HDL-C, while group E had no obvious change. G group can significantly reduce atherosclerosis index (AI, TC/HDL-C), compared with C, E group, the difference was statistically significant (P <0.05). (2) Effect on platelet aggregation: mPAGR was significantly increased in groups A, B, C, and D at 3 days after endothelial injury, and was significantly different from that of N group (P<0.01); G, E, and F All groups were significantly reduced after 3 days of mPAGR, compared with group C, G group differences were statistically significant (P <0.05). G, E, and F in each group can reduce the mPAGR at 6 weeks after operation, which is statistically significant compared with C group (P<0.05, P<0.01). The effect of G group is more significant. Conclusion Qi Capsule can reduce the levels of TC, LDL-C and atherosclerotic index AI in rabbit atherosclerosis model, and also inhibit the platelet aggregation in the early stage of the model and 6 weeks after operation. These may be the mechanism of anti-AS. one.