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目的用不同浓度三七总皂苷(panax notoginseng saponin,PNS)和卡维地洛干预急性心肌梗死(acutemyocardial infarction,AMI)大鼠,评价其改善心功能作用的疗效。方法建立大鼠AMI模型84只,随机分为心肌梗死对照组(AMI组),PNS治疗低(PNS-L,20 mg.kg-1.d-1)、中(PNS-M,40 mg.kg-1.d-1)、高(PNS-H,80 mg.kg-1.d-1)3组,卡维地洛治疗低(CARV-L,2.5 mg.kg-1.d-1)、中(CARV-M,5 mg.kg-1.d-1)、高(CARV-H,10 mg.kg-1.d-1)3组,另设假结扎组(sham组),每组12只。灌胃给药4周后,用小动物超声心动图仪检测心功能,检测血浆心力衰竭标志物N-末端脑钠肽前体(N-terminal pro-brain natriuretic peptide,NT-pro-BNP)浓度,并行心肌组织的苏木素-伊红染色分析。结果同sham组比较,AMI组左心室射血分数和左心室收缩百分率、左心室前壁舒张期厚度、左心室前壁收缩期厚度均明显降低,差异有统计学意义(P<0.01);左心室舒张末期内径﹑左心室收缩末期内径及血浆NT-pro-BNP浓度显著增高,差异有统计学意义(P<0.01)。同AMI组比较,PNS和卡维地洛治疗的中、高剂量组左心室射血分数、左心室收缩百分率、左心室前壁舒张期厚度、左心室前壁收缩期厚度均明显增加,而左心室舒张末期内径和左心室收缩末期内径及血浆NT-pro-BNP浓度明显降低,差异有统计学意义(P<0.01)。而且,各治疗组的心肌组织细胞均得到较好的保护。结论用PNS和卡维地洛治疗AMI大鼠4周均能有效保护心肌组织和改善心功能,减缓心力衰竭的发生、发展,且PNS效果略优。
Objective To investigate the effects of different concentrations of Panax notoginseng saponin (PNS) and carvedilol on acute myocardial infarction (AMI) in rats. Methods Eighty-four AMI rats were randomly divided into 3 groups: AMI group, PNS-M group, PNS-M group and PMS-M group. (CARV-L, 2.5 mg.kg-1.d-1) and carvedilol (PNS-H, 80 mg.kg-1.d-1) (CARV-M, 5 mg.kg-1.d-1) and CARV-H (10 mg.kg-1.d-1) 12 in each group. Four weeks after intragastric administration, heart function was detected by echocardiography, and the concentration of N-terminal pro-brain natriuretic peptide (N-terminal pro-brain natriuretic peptide, , Parallel myocardial tissue hematoxylin-eosin staining analysis. Results Compared with sham group, left ventricular ejection fraction and left ventricular systolic percentage, left ventricular anterior wall diastolic thickness, and left ventricular anterior wall systolic thickness were significantly decreased in AMI group, the difference was statistically significant (P <0.01); left The end-diastolic diameter, left ventricular end-systolic diameter, and plasma NT-pro-BNP concentration were significantly increased, with statistical significance (P <0.01). Compared with AMI group, left ventricular ejection fraction, left ventricular systolic percentage, left ventricular anterior wall diastolic thickness and left ventricular anterior wall systolic thickness were significantly increased in middle and high dose PNS and carvedilol groups, while left The end-diastolic diameter, left ventricular end-systolic diameter, and plasma NT-pro-BNP concentration were significantly lower (P <0.01). Moreover, each treatment group of myocardial tissue cells are better protected. Conclusion Both PNS and carvedilol treatment of AMI rats for 4 weeks can effectively protect myocardial tissue and improve cardiac function, slow down the occurrence and development of heart failure, and the effect of PNS is slightly better.