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以对南方根结线虫(Meloidogyne incognita)表现感病和抗病的新疆野生樱桃李(Prunus sogdiana Vassilcz.)绿枝扦插苗为材料,观察接种南方根结线虫(Meloidogyne incognita)后在侵染数量、巨细胞发育以及CCS52B基因表达方面的差异。结果显示:在感病植株中南方根结线虫的侵染数量明显高于抗病植株。感病植株接种5 d时已可观察到大量线虫建立了取食位点,到21 d线虫已经成熟、产卵并完成其生活史。而在抗病植株中,接种7 d后仅在少数植株中观察到线虫建立了取食位点,到21 d时观察到大量线虫发育停滞,个别线虫到接种后35 d才发育成雌虫。感病植株的巨细胞面积和细胞核面积明显大于抗病植株。CCS52是一类核内周期激活因子,对于核内复制以及依赖于染色体加倍的细胞膨大至关重要。在樱桃李中,CCS52B基因在感病和抗病植株中都只在巨细胞中表达,并且感病植株中早期表达强于抗病植株,说明在感病植株中线虫容易建立取食位点并完成生活史,是由于CCS52B基因参与了巨细胞形成与发育,其早期的高表达提高了巨细胞中的核内复制,促进了细胞核倍性增加,进而促进巨细胞发育从而有利于线虫发育,导致植株感病。
The green shoot cuttings of Prunus sogdiana Vassilcz. From Meloidogyne incognita in China were used to investigate the effects of infection on the number of infected Meloidogyne incognita, Giant cell development and CCS52B gene expression differences. The results showed that the number of infected root-knot nematodes in susceptible plants was significantly higher than that in resistant plants. A large number of nematodes were observed to have established feeding sites on susceptible plants 5 d after inoculation. By 21 d, the nematodes were mature, spawned and completed their life history. In the resistant plants, nematode sites were established in only a few plants 7 days after inoculation, and a large number of nematode development stagnation was observed at 21 days. Individual nematodes developed females 35 days after inoculation. The susceptible plants had significantly larger giant cell area and nucleus area than the resistant plants. CCS52 is a type of nuclear inner-cycle activator that is crucial for nuclear replication as well as cell expansion that is dependent on chromosome doubling. In cherry plum, CCS52B gene is expressed only in giant cells in susceptible and resistant plants, and early expression in susceptible plants is stronger than that in resistant plants, indicating that it is easy for nematodes to establish feeding sites in susceptible plants The completion of the life history is due to CCS52B gene involved in the formation and development of giant cells, the early high expression increased in the giant cell nuclear replication, and promote nuclear ploidy increase, thereby promoting the development of giant cells conducive to nematode development, resulting in Plant disease.