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目的:观察复方鬼箭羽合剂对早期糖尿病肾病的治疗作用,以及探讨这种治疗作用的机制。方法:单侧肾切除STZ腹腔注射造模的方法,采用肾小球光镜和电镜病理染色的方法、以及Smad4和Smad7免疫组织化学染色的方法观察方法复方鬼箭羽合剂对肾小球Smad4和Smad7表达的调控作用。结果:8、12周末模型组大鼠肾小球内Smad4阳性相对面积显著高于正常对照组(P<0.01),复方鬼箭羽合剂治疗组大鼠肾小球内Smad4的蛋白表达量较模型组显著降低(P<0.01),8、12周末模型组大鼠肾小球内Smad7阳性相对面积显著低于正常对照组(P<0.01),复方鬼箭羽合剂治疗组大鼠肾小球内Smad7阳性相对面积与正常对照组差别并不显著(P>0.05),而12周时复方鬼箭羽合剂治疗组大鼠肾小球内Smad7阳性相对面积较8周有所下降,有显著性差异(P<0.05)。结论:复方鬼箭羽合剂治疗组能显著上调Smad4和使Smad7蛋白的表达降低,说明复方鬼箭羽合剂可通过调节Smad4和Smad7蛋白表达,从而降低TGF-β1的表达,达到防治糖尿病肾病肾小球硬化的作用。
Objective: To observe the therapeutic effect of compound ghost arrow febrile mixture on early diabetic nephropathy, and to explore the mechanism of this therapeutic effect. Methods: The method of unilateral nephrectomy STZ intraperitoneal injection method, the use of glomerular light microscopy and electron microscopy pathological staining method, as well as Smad4 and Smad7 immunohistochemical staining method to observe the method of compound ghost arrow fentanyl glomerular Smad4 and Smad7 expression regulation. Results: Compared with the normal control group, the relative area of Smad4 in glomerulus in model group was significantly higher than that in the normal control group (P <0.01) (P <0.01). The relative area of Smad7 in glomeruli in model group was significantly lower than that in normal control group (P <0.01) The relative area of Smad7 positive group was not significantly different from that of the normal control group (P> 0.05). At 12 weeks, the relative area of Smad7 in glomerulus of compound ghost-emuculus treatment group was lower than that of the control group (P <0.05). Conclusion: Compound ghost arrow febrile mixture treatment group can significantly up-regulate Smad4 and Smad7 protein expression reduced, indicating that compound gGG can regulate the expression of Smad4 and Smad7, thereby reducing the expression of TGF-β1, to achieve the prevention and treatment of diabetic nephropathy The role of ball hardening.