论文部分内容阅读
目的 探讨氯原酸 (CHA)、抗坏血酸 (AA)、柠檬酸 (CA)和苹果酸 (MA) 4种有机酸对单核 内皮细胞 (MC EC)粘附作用的影响 ,为开发利用天然食物成分有机酸防治动脉粥样硬化提供科学依据。方法 通过原代培养人脐静脉内皮细胞 (EC) ,观察氧化修饰的低密度脂蛋白 (oxLDL) (10 0mg/L) ,不同剂量 (10、2 0、40mg/L)CHA、AA、CA和MA 4种有机酸 +oxLDL(10 0mg/L) ,以及单纯CHA和AA3个剂量 ,分别与EC共培养 12h后MC EC的粘附率。结果 正常对照组MC EC的基础粘附率是(11 35± 1 2 7) % ;oxLDL组粘附率为 (37 2 5± 5 6 3) % ,显著高于正常组 (P <0 0 1) ;有机酸 +oxLDL各组MC EC粘附率与oxLDL组比较 ,有不同程度的降低 ,其中 3个剂量组的CHA +oxLDL和AA +oxLDL的降低率分别为CHA :6 0 81%、72 6 6 %、10 0 96 % ,AA :34 2 9%、6 5 0 6 %、95 76 % ,呈剂量依赖性 ;CA+oxLDL只有高剂量组的MC EC粘附率显著低于oxLDL组 (P <0 0 5 ) ,MA +oxLDL 3个剂量组无明显作用。单纯CHA和AA各个剂量组的MC EC粘附率略低于正常对照组 ,但差异不显著。结论 CHA和AA对oxLDL诱导的MC EC粘附作用具有明显的抑制效应 ,CA作用较弱 ,而MA作用不明显。
Objective To investigate the effect of four kinds of organic acids (CHA, AA, CC and MA) on the adhesion of monocytes (MCECs) to develop and utilize natural food ingredients Organic acids provide a scientific basis for prevention and treatment of atherosclerosis. Methods Human primary cultured human umbilical vein endothelial cells (ECs) were cultured in vitro. Oxidized low density lipoprotein (oxLDL) (10 0 mg / L), CHA, AA and CA at different doses (10, 20 and 40 mg / L) MA 4 kinds of organic acids + oxLDL (100mg / L), and simple CHA and AA3 doses, EC EC co-cultured with EC for 12h respectively. Results The rate of basal adhesion of MC EC was (11 35 ± 1 27)% in normal control group and (37 2 5 ± 5 6 3)% in oxLDL group, which was significantly higher than that in normal group (P 0 01 ). Compared with oxLDL group, the adhesion rate of MC EC in organic acid + oxLDL group decreased to some extent. The reduction rates of CHA + oxLDL and AA + oxLDL in three dosage groups were CHA: 60 81%, 72 6: 6%, 10: 96%, AA: 34.29%, 65.6%, 95.76% in a dose-dependent manner. The MC EC adhesion rate of CA + oxLDL group was significantly lower than that of oxLDL group P <0 05), MA + oxLDL 3 dose group had no significant effect. The MC EC adhesion rate of CHA and AA alone groups was slightly lower than that of the normal control group, but the difference was not significant. Conclusions CHA and AA have a significant inhibitory effect on oxLDL-induced MC EC adhesion, with a weak effect of CA and a slight effect of MA.