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目的 通过检测T淋巴细胞的细胞周期分布及其细胞周期调节蛋白 (CCRP)的表达 ,探讨发作期支气管哮喘 (简称哮喘 )患者T淋巴细胞过度活化、增殖的调控机制。方法 采用碘化丙啶DNA染色法应用流式细胞仪分析 30例发作期哮喘患者 (哮喘组 )及 2 0名正常人 (正常组 )外周血T淋巴细胞的细胞周期分布 ;采用间接免疫荧光法 ,应用流式细胞仪同步测定相应T淋巴细胞内CCRP、依赖激酶抑制蛋白 (P2 7kipl)、细胞周期蛋白E(cyclinE)、cyclinA、cyclinB的表达水平。比较哮喘患者和正常人上述指标的差异。结果 哮喘组S期、S +G2 /M期的T淋巴细胞百分率分别为 (18± 9) %和(2 5± 10 ) % ,对照组分别为 (5± 4 ) %、(11± 6 ) % ,两组比较差异有显著性 (P均 <0 0 1) ;哮喘组G0 /G1期的T淋巴细胞百分率为 (76± 10 ) % ,对照组为 (90± 6 ) % ,两组比较差异有显著性 (P <0 0 1)。哮喘组T淋巴细胞内P2 7kipl的表达水平为 (4 0± 2 4 ) % ,对照组为 (6 7± 4 8) % ,两组比较差异有显著性(P <0 0 5 ) ;哮喘组cyclinE、cyclinA、cyclinB的表达水平分别为 (2 5± 2 4 ) %、(9± 7) %、(6 4± 5 9) % ,对照组分别为 (6± 5 ) %、(4± 4 ) %、(3 4± 1 6 ) % ,两组比较差异均有显著性 (P均 <0 0 1)。结论 T淋巴细胞?
Objective To investigate the regulatory mechanism of T lymphocyte over-activation and proliferation in patients with bronchial asthma (asthma) by detecting the distribution of cell cycle and the expression of cell cycle regulatory protein (CCRP) in T lymphocytes. Methods The distribution of T lymphocytes in peripheral blood of 30 asthmatic patients (asthma group) and 20 normal subjects (normal group) were analyzed by flow cytometry with propidium iodide DNA staining. The expression of T lymphocytes was detected by indirect immunofluorescence The expression of CCRP, P2 7 kipl, cyclin E, cyclin A and cyclin B in corresponding T lymphocytes were measured by flow cytometry. Comparison of asthma patients and normal subjects above differences. Results The percentages of T lymphocytes in S phase and S + G2 / M phase were (18 ± 9)% and (25 ± 10)% in asthma group and (5 ± 4)% and (11 ± 6) %, The difference between the two groups was significant (P <0.01). The percentage of T lymphocytes in G0 / G1 phase was (76 ± 10)% in asthma group and (90 ± 6)% in control group The difference was significant (P <0.01). The expression level of P2 7kipl in T lymphocytes in asthmatic group was (40 ± 2 4)% and in control group was (67 ± 48)%, there was significant difference between the two groups (P <0.05) The expression levels of cyclin E, cyclin A and cyclin B were (2 5 ± 2 4)%, (9 ± 7)% and (6 4 ± 5 9)% respectively in the control group and (6 ± 5)% and )%, (34 ± 16)% respectively. There was significant difference between the two groups (all P <0 01). Conclusion T lymphocytes?