We cool 85Rb atoms in an integrating sphere directly from a vapor background using diffuse light generated by multiple reflections of laser beams in the inner surface of the integrating sphere. We compare and analyze the different features of cold 85Rb atoms and cold 87Rb atoms in diffuse light cooling, which are important in applying 85Rb and 87Rb isotopes in many experiments on testing fundamental physics. We cool 85Rb atoms in an integrating sphere directly from a vapor background using diffuse light generated by multiple reflections of laser beams in the inner surface of the integrating sphere. We compare and analyze the different features of cold 85Rb atoms and cold 87Rb atoms in diffuse light cooling, which are important in applying 85Rb and 87Rb isotopes in many experiments on testing fundamental physics.