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目的观察镁补充对2型糖尿病大鼠胰岛素受体亲和力的影响。方法将用高脂饮食联合链脲佐菌素(STZ)方法诱发的2型糖尿病大鼠随机分为4个组,糖尿病对照组喂饲高脂饲料,高、中、低剂量组在高脂饲料中分别加入2000、1000、200mg/kg的镁(以镁离子计)。每周检测空腹血糖1次。自由饮食喂养4 w,处死动物。用放射性受体分析法测肝细胞胰岛素受体亲和力、放射免疫法测血清胰岛素(Ins)水平、比色法检测糖化血红蛋白(HbA1c)和血浆丙二醛(MDA)、葡萄糖氧化酶法测空腹血浆葡萄糖(FPG),并计算胰岛素敏感指数(ISI)和抵抗指数(IRI)。结果高剂量组的高亲和力胰岛素受体结合常数(K1)为(4.76±0.08)×108L/mol,低亲和力胰岛素受体结合常数(K2)与结合容量(R2)分别为(1.10±0.14)×106L/mol,(8.49±0.43)×1013/mg蛋白,均较糖尿病对照组显著性升高(P<0.05)。补镁第3 w开始高剂量组空腹血糖较糖尿病对照组显著性降低,高剂量组的胰岛素敏感指数较糖尿病对照组显著性升高,而胰岛素抵抗指数则显著性降低。结论镁补充可以提高2型糖尿病大鼠胰岛素受体亲和力,降低胰岛素抵抗。
Objective To observe the effects of magnesium supplementation on insulin receptor affinity in type 2 diabetic rats. Methods Type 2 diabetic rats induced by high fat diet combined with streptozotocin (STZ) were randomly divided into four groups. Diabetic control rats were fed with high fat diet. The high, middle and low doses of high fat diet Were added 2000, 1000, 200 mg / kg of magnesium (based on magnesium ions). Fasting blood glucose test once a week. Free diet feeding 4 w, the animals were sacrificed. The affinity of insulin receptor was measured by radioactive receptor assay, the level of insulin was measured by radioimmunoassay, the levels of HbA1c and MDA were measured by colorimetry, the fasting plasma glucose Glucose (FPG), and calculate insulin sensitivity index (ISI) and resistance index (IRI). Results The high affinity insulin binding constant (K1) was (4.76 ± 0.08) × 108L / mol and the low affinity insulin binding constant (K2) and binding capacity (R2) were (1.10 ± 0.14) × 106L / mol, (8.49 ± 0.43) × 1013 / mg protein were significantly higher than those in diabetic control group (P <0.05). At the beginning of magnesium supplementation, the fasting blood glucose of high-dose group was significantly lower than that of diabetic control group. The insulin sensitivity index of high-dose group was significantly higher than that of diabetic control group, while the insulin resistance index was significantly lower. Conclusion Magnesium supplementation can improve insulin receptor affinity and reduce insulin resistance in type 2 diabetic rats.