对硅胶电色谱柱的性能进行了考察,发现在水/有机溶剂流动相条件下,几乎不存在气泡问题,流动相的组成在有机溶剂浓度、电解质浓度、pH等方面可以在较大范围变化.选用5种典型样品,对硅胶电色谱的分离机理进行系统研究,发现有反相分配机理、正相吸附机理、离子交换机理以及电泳机理参与作用.同时考察了有机溶剂浓度、电解质浓度、pH值等对分离的影响.此外,还首次提出了一种全新的电色谱模式-动态改性硅胶电色谱. The performance of the silica gel column was investigated. It was found that there was almost no bubble in the mobile phase of water / organic solvent. The composition of the mobile phase could be varied in a wide range of organic solvent concentration, electrolyte concentration and pH. Five kinds of typical samples were selected to study the separation mechanism of silica gel electrochromatography, and the mechanism of reversed-phase partitioning mechanism, positive-phase adsorption mechanism, ion exchange mechanism and electrophoresis mechanism were systematically investigated. The effects of organic solvent concentration, electrolyte concentration, pH value Etc .. In addition, for the first time proposed a new type of electrochromatography - dynamic modified silica gel electrochromatography.