论文部分内容阅读
目的 :观察不同剂量的维拉帕米对Lewis肺癌自发性肺转移的抑制作用。方法 :将Lewis肺癌细胞种植于C57BL/ 6J小鼠右腹股沟皮下 (2× 10 6/鼠 ) ,将 4 0只小鼠随机分成对照组和 3个治疗组 ,从种植第 5天起腹腔内分别注射生理盐水、2 0mg/kg、30mg/kg和 4 0mg/kg维拉帕米 ,每日 1次 ,共 11天 ,第 2 2天处死并解剖小鼠 ,观察对照组和治疗组的肺转移情况 ,体外进行血小板聚集抑制试验。结果 :治疗组肺转移率明显低于对照组 ,随着药物剂量的增加 ,肺转移率逐渐降低 (P <0 0 1) ;维拉帕米可抑制Lewis肺癌细胞诱致的人血小板聚集 ,抑制率随着药物浓度的增加而增加 (P <0 0 1)。结论 :维拉帕米可剂量依赖性地抑制Lewis肺癌自发性肺转移 ,其抗转移作用可能与抑制肿瘤细胞诱致的血小板聚集有关
Objective: To observe the different doses of verapamil on Lewis lung cancer spontaneous lung metastasis. Methods: Lewis lung carcinoma cells were implanted subcutaneously into the right groin (2 × 10 6 / mouse) in C57BL / 6J mice. 40 mice were randomly divided into control group and three treatment groups. From the fifth day after implantation, The rats were injected with physiological saline, verapamil 20 mg / kg, 30 mg / kg and 40 mg / kg verapamil once daily for 11 days. The mice were sacrificed on the 22nd day and dissected to observe the lung metastasis of the control group and the treatment group Situation, in vitro platelet aggregation inhibition test. Results: The lung metastasis rate in the treatment group was significantly lower than that in the control group. With the increase of the drug dose, the lung metastasis rate was decreased (P <0.01). Verapamil could inhibit the human platelet aggregation induced by Lewis lung cancer cells with the inhibition rate Increased with increasing drug concentration (P <0.01). Conclusion: Verapamil can inhibit the spontaneous lung metastasis of Lewis lung cancer in a dose-dependent manner, and its anti-metastatic effect may be related to the inhibition of tumor cell-induced platelet aggregation