Generation of GGTA1 biallelic knockout pigs via zinc-finger nucleases and somatic cell nuclear trans

来源 :中国科学：生命科学英文版 | 被引量 : 0次 | 上传用户：yilvQINGFENG

【摘要】

：

Genetically modified pigs are valuable models of human disease and donors of xenotransplanted organs.Conventional gene targeting in pig somatic cells is extreme

【作者】

：

BAO Lei CHEN HaiDe JONG UiMyon

【机构】

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CollegeofAnimalSciences,InstituteofBiochemistryandCellBiology,DepartmentofAnimalCloning,Departmentof

【出处】

：

中国科学：生命科学英文版

【发表日期】

：

2014年2期

【关键词】

：

体细胞核移植转基因猪基因敲除核酸酶锌指半乳糖基转移酶免疫排斥反应成纤维细胞系 pig xenotransplantation ZFNs GGTA1

【基金项目】

：

supported by grants from Ministry of Science and Technology of China（2011CBA01001,2012AA020503）, the National Science Fund for Distinguished Young Scholars（31025016）, the National Natural Science Foun

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Genetically modified pigs are valuable models of human disease and donors of xenotransplanted organs.Conventional gene targeting in pig somatic cells is extremely inefficient.Zinc-finger nuclease(ZFN)technology has been shown to be a powerful tool for eff

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Generation of GGTA1 biallelic knockout pigs via zinc-finger nucleases and somatic cell nuclear trans

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