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目的:观察蝙蝠葛酚性碱(PAMD)对人胰腺癌细胞BxPC-3裸鼠移植瘤的抑制作用及其病理形态学影响。方法:雌性的裸鼠40只,被接种人源性的胰腺癌细胞株BxPC-3于裸鼠的右腋皮下,主要用于建立人胰腺癌皮下异位移植瘤的实验动物模型。24 h后用随机法将其分为5组,每组8只,分别为PAMD高、低剂量组(20,10 mg·kg-1)5-氟尿嘧啶(5-FU)组(20mg·kg-1),模型组和正常组。PAMD与5-Fu组ip给药,模型组与正常组则给予同体积的生理盐水,但正常组不接种瘤株,给药20 d后,按照相关的公式计算其抑瘤率;在光镜和透射电镜下,观察PAMD对移植瘤组织病理形态学的影响。结果:与正常组比较,模型组瘤重明显增加(P<0.01),肿瘤组织的病理形态和超微结构瘤细胞胞体变大,核浆比例失调,病理性核分裂象增大,核浆比例增大;与模型组比较,PAMD高、低剂量组和5-Fu组对胰腺癌BxPC-3荷瘤裸鼠的瘤组织生长都有一定的抑制作用(P<0.05),其抑瘤率分别为51.67%,37.26%,33.58%,通过光镜和透射电镜显示,PAMD对肿瘤组织的病理形态和超微结构均有一定的改善作用。结论:PAMD能够有效的抑制胰腺癌细胞株Bx PC-3荷瘤裸鼠肿瘤细胞的生长,PAMD高、低剂量组对Bx PC-3荷瘤裸鼠瘤组织的病理形态学变化,具有一定的改善作用。
Objective: To observe the inhibitory effect of PAMD on the growth of human pancreatic cancer BxPC-3 xenografts in nude mice and its pathomorphology. Methods: Forty female nude mice were inoculated with human pancreatic cancer cell line BxPC-3 subcutaneously in the right armpit of nude mice to establish a model animal model of subcutaneous xenograft of human pancreatic cancer. After 24 h, they were randomly divided into 5 groups (8 rats in each group): PAMD high and low dose groups (20 and 10 mg · kg -1) and 5-fluorouracil group (20 mg · kg- 1), model group and normal group. PAMD and 5-Fu groups were administered ip, and the same volume of normal saline was given to the model group and the normal group, but the normal group was not inoculated with the tumor strain. After 20 days of administration, the tumor inhibition rate was calculated according to the relevant formula; And transmission electron microscopy to observe the impact of PAMD on the pathological morphology of xenografts. Results: Compared with the normal group, the tumor weight of the model group increased significantly (P <0.01). The pathological changes of the tumor tissue and the ultrastructure of the tumor cells became larger, the proportion of nuclear plasma increased, the pathological mitosis increased, (P <0.05). Compared with model group, PAMD high, low dose group and 5-Fu group had certain inhibitory effect on the growth of pancreatic cancer BxPC-3 tumor-bearing nude mice (P <0.05) 51.67%, 37.26% and 33.58% respectively. The results of light microscope and transmission electron microscope showed that PAMD could improve the pathology and ultrastructure of tumor tissue. Conclusion: PAMD can effectively inhibit the growth of tumor cells in Bx PC-3 tumor-bearing nude mice. The high and low dose PAMD groups have certain histopathological changes in Bx PC-3 tumor-bearing nude mice, Improve effect