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使用不育昆虫释放技术是一项新兴的苹果蠹蛾Cydia pomonella(L.)防治方法,使用转基因得到不育雄蛾具有比传统辐射方法更多的优点。转基因技术通过使用基于piggyBac等转座子的质粒载体,并插入显性条件致死基因以培育遗传性别品系,同时插入荧光蛋白等标记基因来显示转基因的效果;在人工控制的发育条件,建立成熟的稳定苹果蠹蛾品系,用以最终的田间释放以达到防治的目的。
The use of sterile insect release technology is an emerging Cydia pomonella (L.) control method, the use of transgenic male sterile moths have more advantages than the traditional radiation methods. The transgenic technique shows the effect of the transgene by using a plasmid vector based on a transposon such as piggyBac and inserting a dominant-negative lethal gene to cultivate a genetic lineage while inserting a marker gene such as a fluorescent protein; establishing a mature Stable codling moth line, for the ultimate release of the field in order to achieve the purpose of prevention and treatment.