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目的 :观察不同浓度去甲肾上腺素 ( NA,10 - 1 0 ～ 10 - 6mol/ L )对大鼠 B细胞功能的影响 ,α和β肾上腺素能受体及钙离子通道在 NA影响 B细胞功能中的作用 ,从细胞水平了解 NA对 B细胞功能的调节作用及其作用机制。方法 :利用体外抗体生成的检测方法 ,即用绵羊红细胞 ( SRBC)刺激大鼠肠系膜淋巴结 B细胞转化成抗体形成细胞 ( AFC) ,然后检测其抗体生成量。结果 :( 1)10 - 1 0、10 - 9、10 - 8和 10 - 7mol/ L NA都能显著抑制大鼠 B细胞功能 ,其中 10 - 8mol/ L NA的作用最强 ,而 10 - 6mol/ L NA无明显的抑制作用。( 2 )α受体拮抗剂酚妥拉明 ( phentolamine,10 - 7mol/ L )和β受体拮抗剂心得安 ( propranolol,10 - 7mol/ L )分别作用于淋巴细胞时 ,都不能阻断 NA抑制 B细胞功能的作用 ,而两者共同作用于淋巴细胞时 ,能完全阻断 NA抑制 B细胞功能的作用。 ( 3 )钙通道阻滞剂维拉帕米 ( verapamil,10 - 7mol/ L )和尼群地平 ( nitrendipine,10 - 7mol/ L )都能阻滞 NA抑制 B细胞功能的作用。结论 :NA可非浓度依赖性地抑制大鼠 B细胞功能 ,此作用可能由 B细胞上的α和β肾上腺素能受体共同介导
OBJECTIVE: To observe the effect of norepinephrine (NA, 10 - 10 ~ 10 - 6 mol / L) on the function of B cells in rats. The effects of α and β adrenergic receptors and Ca2 + channels on the B cell function In the role of NA from the cellular level to understand the regulation of B cell function and its mechanism of action. Methods: The method of in vitro antibody production was used to detect the formation of antibody-forming cells (AFCs) in rat mesenteric lymph node B cells stimulated by SRBC. Then the antibody production was detected. Results: (1) 10 - 1, 10 - 9, 10 - 8 and 10 - 7 mol / L NA significantly inhibited the function of B cells in rats, of which 10 - 8 mol / L NA was the most effective, / L NA no significant inhibitory effect. (2) The inhibitory effect of α receptor antagonist (phentolamine (10 - 7mol / L) and β - receptor antagonist propranolol (10 - 7mol / L) on lymphocytes, respectively, Inhibition of B cell function, and the two act together on lymphocytes, can completely block the role of NA inhibit B cell function. (3) The calcium channel blockers verapamil (10 - 7mol / L) and nitrendipine (10 - 7mol / L) blocked the effect of NA on B cell function. CONCLUSION: NA can inhibit B-cell function in a concentration-dependent manner in rats, which may be mediated by α and β adrenergic receptors on B cells