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目的观察褪黑素（MT）对小鼠免疫性肝损伤的影响。方法序贯注射短小棒状杆菌和脂多糖诱导小鼠免疫性肝损伤模型;在造模不同时间注射不同剂量MT;检测血浆转氨酶、肝脏雨二醛（MDA）和谷胱甘肽过氧化物酶（GSH-px）以及脾淋巴细胞增殖状况。结果造模期间用MT（0.1～10.0mg·kg-1）能显著降低血浆转氨酶和肝脏MDA水平（P＜0.05～0.01），使肝脏GSH-px活性部分恢复（P＜0.05），并能选择性抑制刀豆蛋白A诱导的脾淋巴细胞增殖。但造模后使用MT对免疫性肝损伤无明显影响。结论MT保护免疫性肝损伤作用与其抗氧化和免疫调节作用有关。
Objective To observe the effect of melatonin (MT) on immune liver injury in mice. Methods The mice model of immunological liver injury was induced by sequential injection of Corynebacterium parvum and lipopolysaccharide (LPS). Different doses of MT were injected at different time points after modeling. The levels of plasma ALT, liver MDA and glutathione peroxidase GSH-px) and splenic lymphocyte proliferation status. Results MT (0.1 ~ 10.0 mg · kg-1) significantly reduced the levels of plasma transaminase and liver MDA (P <0.05 ~ 0.01) during the modeling period, and partially restored the activity of GSH-px in liver (P <0.05) Inhibitory Concanavalin A Induces Proliferation of Splenic Lymphocytes. However, the use of MT after modeling had no significant effect on immune liver injury. Conclusion The protective effect of MT on immune liver injury is related to its anti-oxidant and immunomodulatory effects.