目的　在烟草中高效表达抑肽酶(bpti)。方法　克隆抑肽酶基因,以烟草叶片为外植体,通过根癌农杆菌将其导入烟草,经卡那霉素抗性筛选和PCR扩增检测基因的整合;抽提转染植株叶片中的抑肽酶,进行活性测定。结果　抗性植株整合并高效表达了抑肽酶。结论　构建的含抑肽酶基因的植物表达载体pBI1 2 1 +bpti具有正确的阅读框,并能在异源植物中表达。 Objective To express aprotinin (bpti) efficiently in tobacco. Methods The aprotinin gene was cloned. Tobacco leaves were used as explants and introduced into tobacco by Agrobacterium tumefaciens. The kanamycin resistance screening and PCR amplification were used to detect the gene integration. Aprotinin, activity assay. As a result, resistant plants integrated and efficiently expressed aprotinin. Conclusion The constructed plant expression vector pBI1 2 1 + bpti containing aprotinin gene has the correct reading frame and can be expressed in heterologous plants.