蟆叶秋海棠(Begonia rex Puts)为美丽的室内观叶植物,常规繁殖系数较低,用花瓣为组培材料,可解决其快繁问题。培养条件取初开的花朵,70％酒精表面灭菌1min,转0.1％升汞灭菌10min,无菌水漂洗3次。剪成0.5×0.5mm的小块,正面向上接种于培养基表面。初代诱导培养基为MS+6-BA2+NAA0.01(单位mg/l,下同),继代(分化、增殖)培养基为MS+6-BA1+NAA0.1,生根培养基为1/2MS+ Begonia rex Puts (Begonia rex Puts) is a beautiful indoor foliage plant. Its conventional breeding coefficient is low, and petal tissue culture material can solve the problem of rapid propagation. Cultivation conditions Take early flowers, 70% alcohol surface sterilization 1min, 0.1% mercuric chloride sterilization 10min, sterile water rinse 3 times. Cut into small pieces of 0.5 × 0.5mm and face-up inoculation on the surface of the medium. Primary induction medium was MS + 6-BA2 + NAA0.01 (unit: mg / l, the same below), and the subculture (differentiation and proliferation) medium was MS + 6- BA1 + NAA0.1. The rooting medium was 1 / 2MS +