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目的评价快速诊断试纸条(DDIA)试剂盒半定量检测方法在血吸虫病流行病学调查中应用的可能性。方法在3个不同流行程度的血吸虫病流行区分别随机选择300、280例和230例当地居民作为检测对象,同时进行粪便病原学检查和用DDIA试剂盒进行抗体水平的半定量检测,通过相关分析评价人群抗体水平和感染率之间的关系。结果人群血吸虫抗体阳性率和抗体几何平均滴度(GRMT)均与粪检阳性率呈直线正相关关系,直线回归方程分别为YI=0.538X-12.470和YG=26.170X-28.761,r分别为0.9579和0.9924(P均<0.05)。结论DDIA试剂盒可以用于血吸虫病流行区人群抗体水平的半定量检测,并以人群GRMT采用直线回归方程(YG=26.170X-28.761)推算出人群感染率的理论值。
Objective To evaluate the feasibility of semi-quantitative detection of rapid diagnostic test strip (DDIA) kit in the epidemiological investigation of schistosomiasis. Methods A total of 300,280 and 230 local residents were randomly selected from three prevalence endemic areas of schistosomiasis for stool etiology and semi-quantitative detection of antibody levels using the DDIA kit. By correlation analysis Evaluate the relationship between population antibody levels and infection rates. Results The positive rate of the antibody and the geometric mean antibody titer (GRMT) of the population were positively correlated with the positive rate of stool examination. The linear regression equations were YI = 0.538X-12.470 and YG = 26.170X-28.761, r were 0.9579 And 0.9924 (P <0.05). Conclusion The DDIA kit can be used for the semi-quantitative detection of antibody levels in schistosomiasis endemic areas. The linear regression equation (YG = 26.170X-28.761) is used to estimate the population infection rate.