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[目的]研究衰老小鼠耐缺氧、应激行为、抗疲劳和神经递质变化,并探讨保健品Oncolyn对衰老的影响。[方法]昆明种小鼠随机分为对照组,衰老组和Oncolyn组。衰老组小鼠颈后注射D-半乳糖42d造成亚急性衰老模型,Oncolyn组在注射D-半乳糖21d后经口给入Oncolyn[0.6g/(kg·d)]70d。应用缺氧、游泳和旷场实验观察动物的行为变化。同时检测脑组织中神经递质去甲肾上腺素和多巴胺的变化。记录体重和脾、胸腺的重量变化。[结果]衰老组的体重比对照组明显下降。衰老组耐缺氧和游泳时间分别为(15.4±3.21)min和(63.4±9.94)min,低于对照组(20.8±4.56)min和(72.3±9.83)min。在旷场实验中,衰老组在中央格停留时间65s、爬格子数34个、站立次数1次和粪便2颗,对照组相应值为8s、51个、3次和1颗,两组差异有显著性。衰老组的脾脏和胸腺的脏器系数与对照组无差异。衰老组脑组织中多巴胺和去甲肾上腺素分别为(1.344±0.127)μg/g和(0.015±0.001)μg/g湿组织,而对照组分别为(1.770±0.129)μg/g和(0.074±0.025)μg/g湿组织,去甲肾上腺素下降较严重。Oncolyn组与衰老组比较体重下降,胸腺的脏器系数增加,脑组织中多巴胺和去甲肾上腺素增加,后者增加了近10倍,在中央格停留时间为18s,爬格数也明显增加,但两组的游泳时间则无差异。[结论]衰老小鼠脑组织神经单胺类递质下降,抗疲劳和耐缺氧能力下降,兴奋反应性降低。On-colyn能提高小鼠对新异环境的应激能力和脑组织中单胺类神经递质,具有一定的延缓小鼠衰老的能力。
[Objective] To study the hypoxia tolerance, stress behavior, anti-fatigue and neurotransmitter changes in senile mice and explore the effect of oncolyn on aging. [Method] Kunming mice were randomly divided into control group, aging group and Oncolyn group. The mice in the aging group were injected with D-galactose for 42 days to induce the subacute aging model. Oncolyn group was given Oncolyn [0.6 g / (kg · d)] for 70 days after D-galactose injection for 21 days. The hypoxia, swimming and open field experiments were used to observe the behavioral changes of animals. At the same time, the changes of neurotransmitters norepinephrine and dopamine in brain tissue were detected. Weight and spleen, thymus weight changes were recorded. [Results] The body weight of the aging group was significantly lower than that of the control group. The hypoxia tolerance and swimming time in the aging group were (15.4 ± 3.21) min and (63.4 ± 9.94) min, respectively, which were lower than those in the control group (20.8 ± 4.56) min and (72.3 ± 9.83) min. In the open-field test, the aging group spent 65s in the central grid, 34 in the grid, 1 times in standing and 2 in stool. The corresponding values in the control group were 8s, 51, 3 and 1, respectively Significance. The organ coefficient of spleen and thymus in the aging group was not different from that in the control group. The levels of dopamine and norepinephrine in the aging group were (1.344 ± 0.127) μg / g and (0.015 ± 0.001) μg / g wet tissue respectively, while those in the control group were (1.770 ± 0.129) μg / g and 0.025) μg / g wet tissue, norepinephrine decreased more serious. Oncolyn group compared with the aging group weight loss, thymus organ coefficient increased, brain tissue dopamine and norepinephrine increased, the latter increased nearly 10 times, in the central lattice stay for 18s, the number of climbing cells also increased significantly, However, there was no difference in swimming time between the two groups. [Conclusion] The monoamine neurotransmitter in the brain of aging mice is decreased, the ability of anti-fatigue and hypoxia-tolerance is decreased and the excitability is decreased. On-colyn can increase the ability of mice to respond to the novel environment and monoamine neurotransmitters in brain tissue, and has certain ability to delay the aging of mice.