于2004年3月从浙江省级中华草龟良种场采集5只乌龟(Chinemys reevesii).参照鱼类的线粒体细胞色素b基因序列设计合成1对引物,扩增了乌龟的细胞色素b基因片段,并对扩增产物进行序列测定分析.结果在5个个体中都获得序列相同的细胞色素b基因片段,长度为408 bp,其中A、T、G、C含量分别为125 bp(30.6%)、113 bp(27.7%)、51 bp(12.5%)、119 bp(29.2%),与其他水生动物相同基因片段碱基序列含量相似. Five turtles (Chinemys reevesii) were collected from the Zhejiang provincial Chinese turtle breeding farm in March 2004. One pair of primers were designed and synthesized according to the sequence of mitochondrial cytochrome b gene in fish, and the cytochrome b gene fragment of turtle was amplified. And the amplified products were sequenced and analyzed.Results Cytochrome b gene fragments with the same sequence were obtained in all 5 individuals, with a length of 408 bp, and the contents of A, T, G and C were 125 bp (30.6%), 113 bp (27.7%), 51 bp (12.5%) and 119 bp (29.2%), which were similar to those of other aquatic animals.