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在灵芝孢子粉的显微鉴定中,对水、甘油醋酸、水合氯醛、95％乙醇等4种试剂的制片效果作了比较,总结出灵芝破壁与不破壁孢子显微鉴定的制片技巧,以和同行商榷。1 材料、仪器与试剂 灵芝孢子粉。电子天平(BP2211北京赛多利斯天平有限公司)生物显微镜(GALEN-Ⅲ型南京江南光电(集团)股份有限公司)。蒸馏水、甘油醋酸、水合氯醛、95％乙醇。
In the microscopic identification of Ganoderma lucidum spores, the effects of four reagents such as water, glycerol acetic acid, chloral hydrate, and 95% ethanol were compared, and the production of microscopic identification of broken and unbroken spores of Ganoderma lucidum was summarized. Skills to discuss with peers. 1 Materials, instruments and reagents Ganoderma spore powder. Electronic Balance (BP2211 Beijing Sartorius Balance Co., Ltd.) Biological Microscope (GALEN-III Nanjing Jiangnan Optoelectronics (Group) Co., Ltd.). Distilled water, glycerol acetate, chloral hydrate, 95% ethanol.