论文部分内容阅读
目的:探讨参莲提取物对脐静脉内皮细胞(HUVEC)功能的影响。方法:建立TNF-α诱导内皮细胞炎性损伤体外模型,采用电镜法检测HUVEC形态学改变,渗透蛋白染色法检测细胞通透性,ELISA法检测细胞分泌功能,激光共聚焦显微镜检测细胞骨架F-actin和胞浆游离钙变化,观察参莲提取物对HUVEC功能的影响。结果:①参莲提取物各剂量组可显著改善HUVEC在炎症因子刺激下细胞结构的改变。②参莲提取物可降低渗透蛋白量,其中1mg/mL组差异有统计学意义(P<0.05)。③参莲提取物各剂量组可明显抑制HUVECF-actin高表达(P<0.05或P<0.01),并呈剂量依赖趋势。④参莲提取物各剂量组可显著抑制HUVEC胞浆游离钙异常增高(均P<0.01)。⑤参莲提取物对HUVEC分泌细胞因子未见明显作用。结论:参莲提取物可有效抑制炎性因子诱导下的内皮细胞结构和功能的改变,呈现出良好的抑制细胞炎性反应作用。
Objective: To investigate the effect of ginseng extract on the function of human umbilical vein endothelial cells (HUVECs). METHODS: TNF-α-induced endothelial cell injury was established in vitro. Morphological changes of HUVEC were observed by electron microscopy. Cell permeabilization was detected by osmotic protein staining. Cell secretory function was detected by ELISA. Confocal laser scanning microscopy was used to detect F- actin and cytoplasmic free calcium to observe the effects of the extract of Senate lotus on the function of HUVECs. Results: ①The ginseng extract could significantly improve the cell structure of HUVEC stimulated by inflammatory cytokines. ② Senate lotus extract can reduce the amount of osmotic protein, 1mg / mL group, the difference was statistically significant (P <0.05). ③Shen lotus extract of each dose group can significantly inhibit the high expression of HUVECF-actin (P <0.05 or P <0.01), and a dose-dependent trend. ④ Shen lotus extract of each dose group can significantly inhibit HUVEC cytoplasmic free calcium anomalies (all P <0.01). ⑤ Senate lotus extract HUVEC secretion of cytokines no significant effect. Conclusion: Shen lotus extract can effectively inhibit the changes of endothelial cell structure and function induced by inflammatory cytokines, showing a good inhibitory effect on cellular inflammatory reaction.