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应用双向凝胶电泳结合质谱鉴定和数据库检索,分析比较C57BL/6J小鼠在多T迷宫(MTM)训练和记忆测试组与未训练组海马蛋白表达的差异,探讨与MTM空间记忆相关的蛋白质.C57BL/6J小鼠经MTM训练后,可对相应的空间线索保持记忆能力,其海马蛋白质表达存在明显差异,14个蛋白质与MTM空间记忆形成显著相关.其中,6个蛋白点表达显著上调,8个蛋白点表达水平显著降低.这些蛋白按功能可分为6类:细胞骨架相关蛋白,物质运输相关蛋白,蛋白合成相关蛋白,能量和物质代谢相关蛋白,信号转导相关蛋白,通道蛋白.这些空间记忆形成相关蛋白的研究深化了对空间记忆机制的认识,为研究和治疗认知相关疾病提供了新靶标.
Two-dimensional gel electrophoresis and mass spectrometry identification and database search were used to analyze the differences of protein expression in C57BL / 6J mice between multi-T-maze (MTM) training and memory test group and untreated group, and to explore the proteins related to spatial memory of MTM. After MTM training, C57BL / 6J mice could retain the memory capacity of the corresponding spatial cues with significant differences in protein expression in the hippocampus, and 14 proteins were significantly associated with the spatial memory of MTM, of which 6 protein spots were significantly up-regulated.8 These proteins are classified into six groups according to their functions: cytoskeleton-related proteins, substance transport-related proteins, protein synthesis-related proteins, energy and substance metabolism-related proteins, signal transduction related proteins, and channel proteins Studies on the formation of related proteins in spatial memory have deepened the understanding of spatial memory mechanisms and provided new targets for the study and treatment of cognitive-related diseases.