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目的:研究载脂蛋白B基因XbaⅠ、EcoRⅠ位点多态性和胆石病之间的关系。方法:通过病例对照研究设计,采用PCR-RFLP技术对106例胆石病患者和105例对照者进行基因分析。结果:胆石病组和对照组ApoB基因XbaⅠ位点X+X-、X-X-基因型构成显著不等;胆石病组X+等位基因频率显著高于对照组(0.104 vs 0.052);胆石病组和对照组ApoB基因EcoRⅠ位点E+E-和E+E+基因型频率构成差异显著,病例组E-等位基因频率显著高于对照组。结论:胆石病发生与ApoB基因XbaⅠ、EcoRⅠ位点多态性存在关联,XbaⅠX+等位基因和EcoRⅠE-等位基因可能为胆石病的易感基因。
Objective: To study the relationship between polymorphisms of Xba Ⅰ and EcoRI sites of apolipoprotein B gene and gallstone disease. Methods: The case-control study design, PCR-RFLP technology in 106 cases of gallstone disease and 105 cases of control subjects for gene analysis. Results: The XbaI genotypes of XbaⅠ locus in gallstone disease group and control group were significantly different. The X + allele frequency in gallstone disease group was significantly higher than that in control group (0.104 vs 0.052) The frequencies of E + E and E + E + genotypes of ApoB gene in control group were significantly different, and the frequency of E-allele in case group was significantly higher than that in control group. Conclusion: The occurrence of cholelithiasis is related to the polymorphism of Xba Ⅰ and EcoR Ⅰ loci of ApoB gene. Xba Ⅰ X + allele and EcoR Ⅰ E-allele may be susceptible gene to gallstone disease.