论文部分内容阅读
目的:测定车前草中总苯乙醇苷及毛蕊花糖苷的含量,为评价药材质量提供科学依据。方法:以毛蕊花糖苷为对照,用紫外-可见分光光度(UV)法测定车前草中总苯乙醇苷的含量,用高效液相色谱(HPLC)法测定毛蕊花糖苷的含量,色谱条件:以色谱柱:ODS2 C_(18)(150 mm×4.6 mm,5μm);流动相:乙腈-0.1%甲酸溶液(13∶87);流速:1 ml·min~(-1),检测波长:332nm,柱温:30℃,进样量:10μl。结果:UV法:对照品溶液和样品溶液在332 nm处有最大吸收,在0.003 1~0.155 0μg·ml~(-1)范围内浓度与吸光度呈良好的线性关系(r=0.999 5),3批样品中总苯乙醇苷的含量分别为2.73%、2.61%、2.84%。HPLC法:毛蕊花糖苷在0.000 6~0.155 0μg·ml~(-1)范围内与峰面积呈良好的线性关系(r=0.999 1),加样回收率为98.5%,RSD为1.6%(n=6),3批样品中毛蕊花糖苷含量分别为0.54%、0.51%、0.56%。结论:该方法操作简单、稳定性好、重复性较好,可用于车前草中总苯乙醇苷及毛蕊花糖苷的含量测定。
Objective: To determine the contents of total phenylethanoid glycosides and verbascoside in plantain and provide a scientific basis for evaluating the quality of medicinal materials. Methods: The content of total phenylethanoid glycosides was determined by UV-Vis spectrophotometry with Verbascoside as control. The chromatographic conditions were determined by high performance liquid chromatography (HPLC) Column: ODS2 C 18 (150 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile-0.1% formic acid solution (13:87); flow rate: 1 ml · min -1; detection wavelength: Temperature: 30 ℃, injection volume: 10μl. Results: UV method: The control solution and the sample solution had the maximum absorption at 332 nm. There was a good linear relationship between the concentration and the absorbance in the range of 0.003 1 ~ 0.155 0 μg · ml ~ (-1) (r = 0.999 5), 3 Total glycosides in batch samples were 2.73%, 2.61% and 2.84%, respectively. HPLC method: Verbascoside showed a good linear relationship with the peak area in the range of 0.0006-0.1550 μg · ml -1 (r = 0.999 1), with a recovery of 98.5% and a RSD of 1.6% (n = 6). The contents of verbascoside in three batches of samples were 0.54%, 0.51% and 0.56%, respectively. Conclusion: The method is simple, stable and reproducible. It can be used for the determination of total phenylethanoid and verbascoside in plantain.