论文部分内容阅读
[Objective]Diffuse reflectance spectrum of near infrared reflectance spectroscopy(NIRS) was investigated for its feasibility to determine the content of stevioside( St) and rebaudioside A( RA) in Stevia rebaudiana leaves. [Methods] The content of St and RA in50 groups of Stevia rebaudiana leaves was determined by high performance liquid chromatography(HPLC). The 50 groups of Stevia rebaudiana leaves were scanned. And PLS was used to compare corrected models established by different spectral pretreatment methods. [Results]Assessing in terms of the correlation coefficients of calibration(rc),the root-mean-square errors of calibration(RMSEC) and prediction(RMSEP),and the residual predictive deviation(RPD). The results of St were 0. 969,3. 42,4. 31 and 3. 85. These of the optimal model for RA were 0. 934,3. 93,4. 33 and 3. 63. The optimal model was obtained by Straight Line Subtraction. [Conclusions]The results indicated that NIRS spectroscopy technique could be used to determine the content of St and RA in Stevia rebaudiana leaves.
[Objective] Diffuse reflectance spectrum of near infrared reflectance spectroscopy (NIRS) was investigated for its feasibility to determine the content of stevioside (St) and rebaudioside A (RA) in Stevia rebaudiana leaves. [Methods] The content of St and RA in50 groups Of Stevia rebaudiana leaves was determined by high performance liquid chromatography (HPLC). The 50 groups of Stevia rebaudiana leaves were scanned. And PLS was used to compare corrected models established by different spectral pretreatment methods. [Results] Assessing in terms of the correlation coefficients of calibration (rc), the root-mean-square errors of calibration (RMSEC) and prediction (RMSEP), and the residual predictive deviation (RPD). The results of St were 0. 969,3. 3. 85. These optimal model was obtained by Straight Line Subtraction. [Conclusions] The results indicated that NIRS spectroscopy technique could be used to determine the conte nt of St and RA in Stevia rebaudiana leaves.