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本文介绍了最新发展的检测植物病毒的3种分子生物学方法,一是直接把植物组织切块点在硝酸纤维膜上,通过免疫反应来检测植物病毒,其优点是早期快速,在间接免疫法中1天可以完成实验,直接免疫法3小时可以完成检测。特异性强,应用了病毒的单克隆抗体后特异性更强,方便易行、准确性高;二是直接把植物组织汁液或提取的病毒核酸点样于硝酸纤维
This paper introduces the latest development of three molecular biology methods for the detection of plant viruses. One is to cut the plant tissue directly on the nitrocellulose membrane. The detection of plant virus by immune reaction has the advantage of early rapidness. In the indirect immunological method In 1 day to complete the experiment, direct immunization method 3 hours to complete the test. Specificity, the application of the monoclonal antibody after the virus more specific, easy to operate, high accuracy; the second is directly to plant tissue juice or extracted from the virus nucleic acid spotted in the nitrocellulose