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目的:比较钠石灰和低压氧仪2种不同缺氧方式诱导的慢性间歇性缺氧小鼠模型的整体行为变化,为进一步构建气阴两虚证动物模型奠定基础。方法:分别采用氧分压从21%逐渐降至6%,平均下降速度为0.5%/min～1%/min的钠石灰缺氧法和氧分压在短时间内骤降后维持在7%～8%的低压氧仪缺氧法,考察2种缺氧模式对小鼠体重,摄食量,自发活动以及心率、T波等气阴两虚临床相关指标变化的影响。结果:钠石灰和低压氧仪2种不同的缺氧方式均能够导致小鼠体重下降;摄食量减少;行走格数减少,直立次数增加等自发活动改变;心率加快,T波持续抬高等心电图变化,二者对各项指标的影响呈基本一致的趋势,其结果具有显著相关性。结论:钠石灰和低压氧仪2种缺氧方式诱导的慢性间歇性缺氧小鼠模型均能够模拟形体消瘦,食欲不振,体倦乏力,烦躁,心悸,脉细数等气阴两虚证的主要临床特征。
OBJECTIVE: To compare the global behavioral changes of chronic intermittent hypoxia mice induced by two different hypoxia modes of sodium lime and hypoxia, which will lay the foundation for the further construction of animal models of deficiency of both qi and yin. Methods: Soda lime anaerobic method and oxygen partial pressure were respectively decreased from 21% to 6% and average descending speed from 0.5% / min to 1% / min respectively, and maintained at 7% ~ 8% low pressure oxygen meter hypoxia method to examine the impact of two hypoxia models on body weight, food intake, spontaneous activity and changes of clinically relevant indexes such as heart rate and T wave. Results: Two different hypoxia modes, sodium lime and hypoxia, could lead to the decrease of body weight, decrease of food intake, change of spontaneous activity such as decrease of walking cell number and increase of upright frequency, change of electrocardiogram . The impact of the two indicators on the indicators is basically the same trend, the results have significant correlation. CONCLUSION: Chronic intermittent hypoxia induced by sodium hypochlorite and hypobaric oxygen can be used to simulate the deficiency of both qi and yin deficiency, such as body weight loss, loss of appetite, body fatigue, irritability, palpitation and pulse breakdown The main clinical features.