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Cu2O/Cu nanoparticles (NPs) in the nanoporous carbon matrix (designated as Cu2O/Cu@NPC) has been synthesized by in-situ calcination of a cupper-based metal-organic framework (Cu-MOF),and its morphology and composition were characterized by PXRD,SEM and Raman.Furthermore,elemental mapping and XPS analysis not only show Cu NPs is generated along with nitrogen (N)-doped carbon,but also indicate Cu2O NPs locates in the extal layer of Cu@NPC.In addition,the adsorption of dye studies implies that Cu2O/Cu@NPC exhibits obvious interaction with Rhodamine B (Rh B) due to the feature of porous and N-doped structure.Cu2O/Cu@NPC has highly electrocatalytic performance for glucose and mercury(Ⅱ) with wide detection range and good stability,which can be used as a novel multifunctional sensor for glucose and mercury(Ⅱ).