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目的：研究粉防己碱（Ｔｅｔ）对大鼠肺动脉平滑肌细胞钙激活钾（ＫＣａ）通道的影响．方法：内面朝外膜片箝单通道记录法．结果：Ｔｅｔ７５和１５μｍｏｌ·Ｌ－１使ＫＣａ的开放概率由０２５１±００１２增加到０３４０±００１３和０４１５±００１１（Ｐ＜００１）．关闭时间由（６１±１５）ｍｓ缩短到（３３±１０）和（２８±１１）ｍｓ（Ｐ＜００１）．Ｔｅｔ３０μｍｏｌ·Ｌ－１使开放概率和开放时间分别降低到（０１１４±０００８）和（１４７±００９）ｍｓ（Ｐ＜０．０１）．结论：Ｔｅｔ对大鼠肺动脉平滑肌细胞ＫＣａ通道有双重作用．
Objective: To investigate the effect of tetrandrine on calcium-activated potassium (KCa) channels in rat pulmonary artery smooth muscle cells. Method: Single-channel recording with inner-facing patch-clamp. Results: Tet7.5 and 15μmol·L-1 increased the open probability of KCa from 0251 ± 0012 to 0340 ± 0013 and 0415 ± 0011 (P <001). Closing time was reduced from (61 ± 15) ms to (33 ± 10) and (28 ± 11) ms (P <001). Tet30μmol·L-1 decreased the open probability and open time to (0114 ± 0008) and (147 ± 009) ms respectively (P <0.01). CONCLUSION: Tet has a dual effect on KCa channel in rat pulmonary artery smooth muscle cells.