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目的:比较清开灵组分黄芩苷(BA),栀子苷(JA),胆酸(CA)与珍珠母(CM)在治疗海马缺血过程中基因表达变化机制。方法:75只6周龄小鼠随机分为BA,JA,CA,CM,M(模型组),每组15只,建立海马缺血再灌注小鼠模型,2 h后实验组分别经尾静脉按3 mL.kg-1体重注射相应药物,24 h后断头取脑制作冠状切片,TTC(氯化四唑)染色,计算不同组别梗死体积百分比;同时抽提小鼠海马组织的总RNA,利用与脑缺血相关的374个基因的cDNA芯片检测基因表达谱变化,采用Ar-raytrack软件选取P<0.05,Fold change>1.5的差异基因,根据差异基因的GO功能探讨药效作用特点。结果:除CM组外,BA,JA,CA与模型组比较均能有效减少海马缺血梗塞面积(P<0.05)。BA,JA,CA与CM比较差异基因数量分别为41条(上调24,下调17),22条(上调13,下调9),11条(上调8,下调3),它们共同抑制Myb基因的表达。结论:BA,JA,CA产生药效结果不仅有共同的基因作用靶点和作用机制,同时还存在多样性的特点。
OBJECTIVE: To compare the mechanism of the changes of gene expression of Qingkailing, including baicalin (BA), jasinosid (JA), cholic acid (CA) and mother of pearl (CM) Methods: Seventy-six mice of 6 weeks old were randomly divided into BA, JA, CA, CM and M groups (model group), with 15 mice in each group. The model of hippocampal ischemia / reperfusion was established. The animals were injected with 3 mL.kg-1 body weight, 24 h later, the brains were cut out to make coronal sections and TTC (tetrazolium chloride) staining to calculate the percentage of infarct volume in different groups. At the same time, total RNA was extracted from hippocampus of mice , 374 genes related to cerebral ischemia were detected by cDNA microarray. The gene expression profiles of P <0.05 and Fold change> 1.5 were detected by Ar-raytrack software, and the pharmacodynamic effects were analyzed according to the GO function of the differentially expressed genes. Results: Compared with the model group, all the BA, JA and CA groups could effectively reduce the area of ischemic infarction in the hippocampus (P <0.05). The number of differentially expressed genes in BA, JA, CA and CM were 41 (up 24, down 17), 22 (up 13, down 9) and 11 (up 8, down 3), respectively, . Conclusion: The results of pharmacodynamics of BA, JA and CA not only have common gene target and mechanism, but also have the characteristics of diversity.