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目的 探讨多原核合子经显微穿刺纠正后的发育情况。方法 将 1999年 9～ 12月行体外受精 胚胎移植中发现的 31例患者的多原核合子 ,在倒置显微镜下 ,用显微穿刺针将多原核合子中多余的原核抽出来 ,之后继续培养 ,观察其卵裂细胞数 ;并与同时期的正常前期胚胎相比较。结果在 37℃条件下培养 2 4h后 ,31例多原核合子中有 17例 (5 5 % )发育到 2～ 4细胞前期胚胎。与正常合子的分裂率 94% (2 9/ 31)相比 ,差异有显著性 (P <0 0 5 )。但 48h后两者前期胚胎的分裂速度间差异无显著性 (P >0 0 5 )。结论 用显微抽吸法纠正多原核合子可能会影响部分合子的分裂 ,但其余合子仍能正常发育
Objective To investigate the development of polyprotocolon after micro-puncture. Methods The polyprotocolon of 31 patients found during in vitro fertilization embryo transfer from September to December in 1999 was extracted with the microscopic needle and the extra pronucleus of polyprotocolon was extracted under an inverted microscope and then cultured and observed The number of cleavage cells; and compared with the same period of normal early embryos. Results After culturing for 24 hours at 37 ℃, 17 (55%) of 31 polyprototic nuclei developed into 2-4 preeclampsia embryos. Compared with the normal cleavage rate 94% (29/31), the difference was significant (P <0 05). However, there was no significant difference in the rate of cleavage between the pre-embryos at 48h (P> 0.05). Conclusions It is suggested that the use of micro-aspiration to correct multiprotocolon may affect the distribution of some zygotes, but the rest of the zygotes can still develop normally