以壳聚糖与β-环糊精为原料、高碘酸钠为氧化剂、环氧氯丙烷为交联剂,用反相悬浮法制备新型壳聚糖/β-环糊精,用红外光谱和电镜扫描对其进行结构表征,研究其对葛根素的吸附性、洗脱性及重复使用性.结果表明,最佳吸附条件为:吸附时间4h,温度35℃,pH=7,葛根素初始浓度0.5mg/mL,壳聚糖/环糊精对葛根素的吸附量达52.43mg/g,洗脱率达89.69%,且表现出较好的重复使用性. Using chitosan and β-cyclodextrin as raw materials, sodium periodate as oxidant and epichlorohydrin as crosslinking agent, a new type of chitosan / β-cyclodextrin was prepared by reversed-phase suspension method. The results showed that the optimal adsorption conditions were as follows: adsorption time 4h, temperature 35 ℃, pH = 7, the initial concentration of puerarin 0.5mg / mL. The adsorption amount of puerarin by chitosan / cyclodextrin reached 52.43mg / g, the elution rate reached 89.69%, and showed good reusability.