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目的 探讨雌激素对大鼠视网膜缺血再灌注所致视网膜损伤的保护作用。 方法 60只去势雌性SD大鼠随机分为2组,行前房灌注,建立视网膜缺血再灌注(RIR)模型。实验组在升高眼压前2h按10 0 μg/kg的剂量皮下注射17β雌二醇。对照组大鼠皮下注射等量生理盐水。分别在灌注前,再灌注后12、2 4、48、72h对视网膜进行常规HE染色切片,观察细胞丢失情况及测量视网膜内层厚度。采用末端脱氧核酸转移酶介导的脱氧三磷酸尿苷缺口末端标记法(TUNEL法)检测视网膜组织中凋亡细胞的表达。 结果 实验组再灌注后2 4、48h的凋亡细胞数目明显低于对照组(P <0 .0 5 ) ,光学显微镜下计数视网膜神经节细胞数较对照组多(P <0 .0 5 )。 结论 雌激素对缺血再灌注所导致的视网膜损伤具有保护作用。
Objective To investigate the protective effect of estrogen on retinal damage induced by retinal ischemia-reperfusion in rats. Methods Sixty ovariectomized female Sprague-Dawley rats were randomly divided into two groups. The anterior chamber perfusion was performed to establish the retinal ischemia-reperfusion (RIR) model. In the experimental group, 17β-estradiol was injected subcutaneously at a dose of 100 μg / kg 2 h before the intraocular pressure was increased. Control rats were injected subcutaneously with normal saline. Retinal HE staining was performed on the retina 12, 24, 48, and 72 hours before and after perfusion to observe the cell loss and the thickness of the retina. TUNEL method was used to detect the expression of apoptotic cells in retinal tissue by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). Results The number of apoptotic cells in the experimental group at 2, 448h after reperfusion was significantly lower than that in the control group (P <0.05). The number of retinal ganglion cells in the experimental group was more than that in the control group (P <0.05) . Conclusion Estrogen has a protective effect on retinal damage induced by ischemia-reperfusion.