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目的对从广州白云国际机场口岸入境的一例自苏里南归国发热人员进行寨卡病毒实验室检测。方法采集发热人员的血液和唾液样本,同时使用两种寨卡病毒实时荧光RT-PCR试剂进行寨卡病毒核酸检测。RT-PCR扩增寨卡病毒部分基因片段,并进行序列测定和同源性比较。基于扩增片段构建系统进化树,分析输入性病例的来源。结果实时荧光RT-PCR结果显示,该病例血清样本寨卡病毒核酸弱阳性、唾液样本寨卡病毒核酸阳性。RT-PCR扩增出预期大小约1.5Kb片段,测序结果表明该片段与巴西寨卡病毒株(GenBank号KX197250)相应序列的同源性为99%。系统进化树显示该病毒属亚洲谱系。结论根据患者流行病学史、临床表现和病例标本核酸检测情况,确诊该病例为广东首例从苏里南地区输入性寨卡病例。
Objective To test a case of Zika virus virus entering Suriname from Suriname from a port entering Guangzhou Baiyun International Airport. Methods The samples of blood and saliva from fever were collected, and two Zika virus real-time RT-PCR reagents were used to detect the Zika virus nucleic acid. RT-PCR amplification of Zika virus part of the gene fragment, and sequencing and homology comparison. Based on the amplified fragment, phylogenetic tree was constructed to analyze the source of imported cases. Results The real-time RT-PCR results showed that the serum samples were weakly positive for Zika virus nucleic acid and saliva samples were positive for Zika virus. RT-PCR amplification of the expected size of about 1.5Kb fragment, sequencing showed that the fragment and the Brazilian Zika virus strain (GenBank No. KX197250) corresponding sequence homology was 99%. The phylogenetic tree shows that the virus belongs to Asian pedigree. Conclusion According to the patient’s epidemiological history, clinical manifestations and case detection of nucleic acid samples, the case was confirmed as Guangdong’s first case of imported Zika from Suriname.