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目的探讨镧对大鼠神经行为影响及海马组织氨基酸类神经递质释放的改变。方法健康成年雌性Wistar大鼠24只,随机分为对照组及低、中、高剂量染镧组(0.25%、0.50%、1.00%La Cl3),仔鼠自母鼠怀孕至断乳后1个月饮用不同剂量氯化镧;穿梭箱实验检测仔鼠神经行为改变,高效液相色谱法检测仔鼠海马中兴奋性氨基酸递质及抑制性氨基酸递质含量。结果与对照组比较,中、高剂量染镧组仔鼠接受电击时间、主动逃避潜伏期时间[分别为(26.00±8.11)、(36.38±5.88)与(6.50±2.45)、(9.38±3.50)s]]均延长,差异有统计学意义(P<0.05),表现出学习记忆损伤;与对照组比较,中、高剂量染镧组仔鼠海马兴奋性氨基酸递质谷氨酸(Glu)、天冬氨酸(Asp)含量[分别为(8.16±2.16)、(12.05±4.86)与(8.91±3.48)、(12.33±4.57)μmol/gpro]升高,中、高剂量染镧组仔鼠海马抑制性氨基酸递质甘氨酸(Gly)、γ-氨基丁酸(GABA)、牛磺酸(Tau)含量[分别为(9.95±3.41)、(13.25±5.03)、(10.93±3.53)、(14.50±4.90)与(10.10±3.18)、(13.78±5.28)μmol/gpro]升高,差异有统计学意义(P<0.05);呈剂量效应关系。结论亚慢性镧染毒可导致仔鼠学习记忆能力下降,其机制可能与仔鼠海马组织内兴奋性及抑制性氨基酸神经递质含量升高有关。
Objective To investigate the effect of lanthanum on the neurobehavioral changes and the changes of amino acid neurotransmitters in hippocampus of rats. Methods Twenty-four healthy adult female Wistar rats were randomly divided into control group and low, middle and high dose lanthanum group (0.25%, 0.50%, 1.00% LaCl3) Different doses of lanthanum chloride were consumed in a month. The shuttle test was used to test the neurobehavioral changes of the pups. The contents of excitatory amino acids and inhibitory amino acids in the hippocampus were detected by high performance liquid chromatography. Results Compared with the control group, the medium and high doses of lanthanum-loaded group received shock time and were able to avoid the incubation period [(26.00 ± 8.11), (36.38 ± 5.88) and (6.50 ± 2.45), (9.38 ± 3.50) s ]] Were prolonged, the difference was statistically significant (P <0.05), showing learning and memory impairment; compared with the control group, medium and high doses of lanthanum group hippocampus excitatory amino acid transmitter glutamate (Glu), day (8.16 ± 2.16), (12.05 ± 4.86) and (8.91 ± 3.48) and (12.33 ± 4.57) μmol / gpro], respectively. The hippocampus of hippocampus The contents of glycine, GABA and Tau in inhibitory amino acids [(9.95 ± 3.41), (13.25 ± 5.03), (10.93 ± 3.53) and (14.50 ± 4.90) and (10.10 ± 3.18), (13.78 ± 5.28) μmol / gpro], the difference was statistically significant (P <0.05); the dose-response relationship. Conclusion Subchronic exposure to lanthanum can reduce learning and memory ability of pups, which may be related to the increase of excitability and inhibitory amino acid neurotransmitters in the hippocampus.