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目的：获取较纯的微量胃癌细胞并检测ｐ５３基因的突变。方法：在显微镜下，直接分离出胃癌组织中微小癌灶内细胞，并应用单链构象多态（ＳＳＣＰ）技术分析该微量癌细胞中ｐ５３基因的突变。结果：在１７１例胃癌组织的石蜡标本中，有 ７０例获得可靠结果包括 ６例早期胃癌和 ６４例进展期胃癌，该方法总成功率为 ４１.０％。结论：显微分离细胞技术十分有利于观察癌前病变、原位癌及浸润癌的基因变化；该方法直接证明早期胃癌细胞中ｐ５３基因发生了突变。
Objective: To obtain more pure trace gastric cancer cells and to detect the mutation of p53 gene. Methods: The microvessel of gastric cancer tissue was directly isolated under microscope. The single nucleotide polymorphism (SSCP) was used to analyze the mutation of p53 gene in this micro - cancer cell line. RESULTS: Of the 171 paraffin-embedded specimens of gastric cancer, 70 had reliable results including 6 early gastric cancers and 64 advanced gastric cancers, with a total success rate of 41.0%. CONCLUSION: Microdissected cells technique is very useful for observing the gene changes of precancerous lesion, carcinoma in situ and invasive carcinoma. This method directly demonstrates the mutation of p53 gene in early gastric cancer cells.