论文部分内容阅读
以能源植物南荻的幼穗为外植体,MS为基本培养基,通过在诱导培养基中分别添加一定浓度梯度的聚乙烯吡咯烷酮(polyvinylpyrrolidone,PVP)、维生素C(vitamin C)及柠檬酸(citric acid,CA),对比研究3种抗褐化剂对南荻外植体褐变及愈伤诱导率的影响。结果表明:在诱导培养基中,添加PVP、维生素C和CA均能减轻外植体的褐化程度;相对于对照(CK)35.83%的诱导率,在试验浓度范围内,添加PVP、维生素C和CA后愈伤组织诱导率可分别提高至55.42%、50.42%和124.00%,CA处理组与对照组之间愈伤诱导率差异达极显著水平(P<0.01)。因此,CA为南荻幼穗愈伤组织诱导的最佳抗褐化剂,且浓度为0.2g/L时诱导率显著提高2.46倍。
Using the young ear of Nandi as explants and MS as the basic medium, polyvinylpyrrolidone (PVP), vitamin C (citric acid) and citric acid acid, CA) were used to study the effects of three browning agents on the browning and callus induction of Nandi explants. The results showed that addition of PVP, vitamin C and CA could reduce the degree of browning of explants in the induction medium. Compared with the induction rate of 35.83% of control (CK), in the experimental concentration range, adding PVP, vitamin C And callus induction rate after CA increased to 55.42%, 50.42% and 124.00% respectively. The difference of callus induction rate between CA treatment group and control group reached extremely significant level (P <0.01). Therefore, CA is the best browning agent induced by young apple callus, and the induction rate increased 2.46 times when the concentration was 0.2g / L.