论文部分内容阅读
测量血小板大小是估计血小板成熟的有用方法,已用于评价临床所出现的有关血小板减少症和/或血小板增多症。本文所述的是一种简便而快速的方法。方法:以EDTA抗凝的血作涂片,进行普通染色。并备有含聚乙烯微小珠的水性乳剂,每毫升乳剂至少有10~7个微小珠,珠的大小为直径2.02微米和3.5微米二种。取0.03毫升乳剂加入0.5毫升水中,充分摇动混匀,即从中取一滴(0.03毫升)涂布薄薄的一层于上述染色的血片上,置室温内待干,在显微镜的油镜视野下进行血小板与珠大小的对比,以测量血小板大小。分三类大小进行记录,即≤2.02微米;>2.02<3.5微米;≥3.5微米。共测量500个血小板大小,以各类大小所占的百分数表示之。
Measuring platelet size is a useful method of assessing platelet maturation and has been used to evaluate clinically relevant thrombocytopenia and / or thrombocythemia. This article describes a quick and easy method. Methods: EDTA anticoagulant blood for smear, ordinary staining. And with polyethylene micro beads containing water-based emulsion per milliliter of emulsion at least 10 to 7 micro-beads, the size of the beads 2.02 microns in diameter and 3.5 microns two kinds. Take 0.03 ml of the emulsion into 0.5 ml of water and shake well to obtain a drop of 0.03 ml. Apply a thin layer of the above stained blood to the plate, leave to room temperature and dry under a microscope oil-field Platelet to bead size comparison to measure platelet size. Record in three sizes, ie ≤ 2.02 μm;> 2.02 <3.5 μm; ≥ 3.5 μm. A total of 500 platelet sizes were measured, expressed as a percentage of various sizes.