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目的:本研究通过检测肠易激综合征(IBS)患者血清中FNF-α和IL-8的含量,旨在通过观察它们在IBS患者中的变化来窥探IBS发病机制中有无炎症免疫因素参与,从而进一步探讨能否从改善机体免疫力的角度来治疗IBS。方法:应用酶联免疫吸附试验(ELISA)方法(ABC法)检测了32例腹泻型IBS及18例便秘型IBS患者血清中的TNF-α和IL-8的含量。结果:(1)正常对照组、便秘型IBS患者血清内可测不到TNF-α,腹泻型IBS患者血清内测到低浓度的TNF-α。(2)腹泻型IBS患者血清IL-8含量显著高于正常(P<0.05),便秘型IBS患者血清内IL-8水平略高于正常对照组,但差异无显著性(P>0.05)。(3)相关分析表明,腹泻型IBS患者血清TNF-α与IL-8水平呈正相关(r=0.928,P<0.01)。结论:(1)腹泻IBS患者血清内TNF-α和IL-8含量均较正常升高,提示腹泻型IBS有炎症因子活化。(2)例秘型IBS患者TNF-α、IL-8与正常无显著差异,提示便秘型IBS和腹泻型IBS的免疫紊乱有着不同的病理生理机制。(3)腹泻型IBS患者IL-8的分泌伴随着TNF-α的增多,TNr-α可能通过活化NF-kB来刺激IL-8的分泌。
Objective: In this study, by detecting the content of FNF-α and IL-8 in the serum of patients with irritable bowel syndrome (IBS), we aimed to explore whether there are inflammatory factors involved in the pathogenesis of IBS by observing their changes in IBS patients , To further explore whether it can improve the body’s immune system to treat IBS. Methods: Serum levels of TNF-α and IL-8 in 32 cases of diarrhea-type IBS and 18 cases of constipation-type IBS were detected by enzyme linked immunosorbent assay (ELISA) method (ABC method). Results: (1) TNF-α was undetectable in the serum of constipation-type IBS patients and normal controls, but low concentrations of TNF-α were detected in the serum of IBS patients with diarrhea. (2) Serum IL-8 levels in IBS patients with diarrhea were significantly higher than those in normal controls (P <0.05). Serum levels of IL-8 in patients with constipation-type IBS were slightly higher than those in normal controls (P> 0.05). (3) Correlation analysis showed that there was a positive correlation between serum TNF-α and IL-8 in patients with diarrhea-type IBS (r = 0.928, P <0.01). Conclusions: (1) Serum levels of TNF-α and IL-8 in IBS patients with diarrhea are higher than normal, suggesting that inflammatory factors are activated in diarrhea-predominant IBS patients. (2) TNF-α and IL-8 in patients with secretory IBS were not significantly different from normal, suggesting that the immune disorders of constipation-like IBS and diarrhea-like IBS have different pathophysiological mechanisms. (3) The secretion of IL-8 in patients with diarrhea-type IBS is accompanied by the increase of TNF-α, and TNr-α may stimulate the secretion of IL-8 by activating NF-κB.