目的探讨应用6-羟基多巴胺(6-OHDA)毁损大鼠黑质致密部制作偏侧帕金森病(PD)模型的方法和应用价值。方法采用立体定向微量注射6-OHDA于大鼠黑质致密部,观察经阿朴吗啡诱导后大鼠的行为及黑质多巴胺能神经元形态学变化。结果部分大鼠注射后即出现行动迟缓、少动、竖毛、躬身、尾部强直、肢体震颤、嗅探和易激惹等异常行为。术后4周时,共33只大鼠经阿朴吗啡诱导后在30min(P<0.01)的平均旋转圈数>7 r/min,达到成功模型的标准,模型成功率为82.5%(33/40)。免疫组化观察发现模型组大鼠注射侧黑质区多巴胺能神经元较对侧和对照组注射侧区明显减少(P<0.01)。结论利用6-OHDA毁损大鼠黑质致密部可以较快建立稳定的PD大鼠模型,方法简便实用,动物死亡率低,模型成功率高。 Objective To investigate the method and application of 6-hydroxydopamine (6-OHDA) -based rat model of substantia nigra compactness in making Parkinson’s disease (PD) model. Methods Microinjection of 6-OHDA into the substantia nigra pars compacta was performed by stereotactic injection. The morphological changes of dopaminergic neurons in substantia nigra were observed after apomorphine induction. Results Some rats showed abnormal behavior such as slow movement, less movement, vertical hair, bending, tonic ankle, limb tremor, sniffing and irritability after injection. At 4 weeks after operation, a total of 33 rats were apomorphine-induced, and the mean number of rotations was> 7 r / min at 30 min (P <0.01). The success rate was 82.5% (33 / 40). Immunohistochemistry showed that dopaminergic neurons in the substantia nigra of injection group were significantly decreased compared with the contralateral group and the control group (P <0.01). Conclusion The rat substantia nigra degeneration damaged by 6-OHDA can establish a stable PD rat model quickly and easily. The method is simple and practical, animal mortality rate is low, and model success rate is high.