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活体增殖和体外组织培养增殖的BsNPV悬液稀释成不同浓度,分别喷于人工饲料上,使其自然渗入饲料内,接3龄幼虫取食感染,感染剂量与幼虫死亡率的回归直线方程分别为;y=3.1815+0.679x和y=3.2000+0.665x,由回归直线方程推算的LD_(50)值及95％置信界限为4.0753×10~2[9.6075×10~2][1.7301×10~2]PIB/克饲料及5.0898×10~2[1.2411×10~3][2.0874×10~2]PIB/克饲料。感染剂量为4×10~5PIB/克饲料的LT_(50)值分别为4.7168天和4.8083天。比较两种来源的BsNPV,其多角体的感染力无明显差异。
BsNPV suspensions proliferated in vitro and proliferated in vitro were diluted to different concentrations and sprayed on the artificial diet respectively to naturally infiltrate the feed. The linear regression equations of feeding instars and larval mortality of the 3rd instar larvae were ; y = 3.1815 + 0.679x and y = 3.2000 + 0.665x. The LD_ (50) value and the 95% confidence bound of the regression linear equation are 4.0753 × 10 ~ 2 [9.6075 × 10 ~ 2] [1.7301 × 10 ~ 2 ] PIB / g feed and 5.0898 × 10 ~ 2 [1.2411 × 10 ~ 3] [2.0874 × 10 ~ 2] PIB / g feed. The LT_ (50) values of infection dose of 4 × 10 ~ 5PIB / g feed were 4.7168 days and 4.8083 days respectively. Comparing BsNPV from two sources showed no significant difference in infectivity between polyhedra.